Thanks to all the speakers, organisers and everyone who came to our World One Health Day for an enjoyable event. Below are some pictures from the day.
See you all next year!
Where: St Mary’s Building, 190 Myers Street, Geelong, 3220
All welcome at this free event.
Please click here for Program details
Please feel free to drop in to one, two or all three sessions! To ensure adequate catering you can register at eventbrite
Brief summary of the paper: We present an optimised metagenomics method for detection and characterisation of all virus types including single and double stranded DNA/RNA and enveloped and non-enveloped viruses.
Initial evaluation included both spiked and non-spiked bird faecal samples as well as non-spiked human faecal samples. From the non-spiked bird samples (Australian Muscovy duck and Pacific black ducks) we detected 21 viruses, and we also present a summary of a few viruses detected in human faecal samples.
We then present a detailed analysis of selected virus sequences in the avian samples that were somewhat similar to known viruses, and had good quality (Q20 or higher) and quantity of next-generation sequencing reads, and was of interest from a virological point of view, for example, avian coronavirus and avian paramyxovirus 6. Some of these viruses were closely related to known viruses while others were more distantly related with 70% or less identity to currently known/sequenced viruses.
Besides detecting viruses, the technique also allowed the characterisation of host mitochondrial DNA present and thus identifying host species, while ribosomal RNA sequences provided insight into the “ribosomal activity microbiome”; of gut parasites; and of food eaten such as plants or insects, which we correlated to non-avian host associated viruses.
A GCEID team has discovered coronaviruses in Australia’s wild birds, showing the nation is not as isolated from potentially serious host-jumping virus outbreaks as previously thought. The researchers, led by the Geelong Centre for Emerging Infectious Diseases (GCEID), has discovered that two different types of coronaviruses are present in Australian wild birds. The discovery marks an important step in building scientists’ understanding of this family of viruses, which have a history of being able to jump into new host species and cause disease.
Read more on this @ Deakin Invenio: Virus discovery in Australian wild birds
The study is the first to demonstrate the presence of coronaviruses in Australian wild birds, and was published in “Nature: Scientific Reports”. See below for more details:
Brief summary of the paper: We evaluated the presence of coronaviruses by PCR in 918 Australian wild bird samples collected during 2016–17. Coronaviruses were detected in 141 samples (15.3%) from species of ducks, shorebirds and herons and from multiple sampling locations.
Sequencing of selected positive samples found mainly gammacoronaviruses, but also some deltacoronaviruses. The detection rate of coronaviruses was improved by using multiple PCR assays, as no single assay could detect all coronavirus positive samples. Sequencing of the relatively conserved Orf1 PCR amplicons found that Australian duck gammacoronaviruses were similar to duck gammacoronaviruses around the world.
Some sequenced shorebird gammacoronaviruses belonged to Charadriiformes lineages, but others were more closely related to duck gammacoronaviruses. Australian duck and heron deltacoronaviruses belonged to lineages with other duck and heron deltacoronaviruses, but were almost 20% different in nucleotide sequence to other deltacoronavirus sequences available. Deltacoronavirus sequences from shorebirds formed a lineage with a deltacoronavirus from a ruddy turnstone detected in the United States.
Given that Australian duck gammacoronaviruses are highly similar to those found in other regions, and Australian ducks rarely come into contact with migratory Palearctic duck species, we hypothesise that migratory shorebirds are the important vector for moving wild bird coronaviruses into and out of Australia.